Multimode Microplate Reader with Advanced Detection Capabilities
- Luminescence, fluorescence, UV-visible absorbance, BRET and FRET detection
- 6- to 384-well microplate reader
- Optimized with Promega cell viability and genetic reporter assays
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GloMax® Discover Microplate Reader
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Easy-to-Use Plate Reader
GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. This advanced plate reader provides flexible use of filters and includes high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. GloMax® Discover can be used as a standalone plate reader or integrated into high-throughput automated workflows. Results are easy to interpret using integrated data analysis software.
![GloMax® Discover Microplate Reader | Plate Reader (4) GloMax® Discover Microplate Reader | Plate Reader (4)](https://i0.wp.com/scv10mr-cdnpre-t-cus-00.azureedge.net/-/media/images/products/fluorometers-luminometers-multimode-readers/multimode-readers/glomax-discover/gm3000-microplate-reader-luminescence-detection.png?rev=1002e01f12ae4f06bd09c8af2c0696cc&sc_lang=it-it)
Quick Results from Lab Friendly Microplate Readers
Performance
GloMax® Discover multimode microplate reader delivers maximum flexibility with minimal user intervention and can be integrated into low- and medium-throughput automation workflows. The instrument provides superior luminescence sensitivity and dynamic range for both strong and weak bioluminescence experimental samples, as well as seamless integration with Promega bioluminescent cell and gene reporter assays.
![GloMax® Discover Microplate Reader | Plate Reader (6) GloMax® Discover Microplate Reader | Plate Reader (6)](https://i0.wp.com/scv10mr-cdnpre-t-cus-00.azureedge.net/-/media/images/products/fluorometers-luminometers-multimode-readers/multimode-readers/glomax-discover/gm3000-glomax-multimode-microplate-reader.jpg?rev=21bfa277c2bf4285ba9e177ae3cdc559&sc_lang=it-it)
- Simple touch screen navigation with full PC capabilities
- Choose from over 50 preloaded protocols and spend less time optimizing
- Build custom protocols using the intuitive drag-and-drop interface
- Export data to a network, cloud or any drive desired
![GloMax® Discover Microplate Reader | Plate Reader (7) GloMax® Discover Microplate Reader | Plate Reader (7)](https://i0.wp.com/scv10mr-cdnpre-t-cus-00.azureedge.net/-/media/images/products/fluorometers-luminometers-multimode-readers/multimode-readers/glomax-discover/gm3000-microplate-reader-luminescence-detection.jpg?rev=0abb1c270de74df792a65aac9741ef09&sc_lang=it-it)
- Filter paddles and automatic filter switching allow easy two-color multiplexing assays or kinetic studies
- Quick read-speeds for high efficiencies in your laboratory
- Compatible for 3rd party automation control and data export to LIMS
![GloMax® Discover Microplate Reader | Plate Reader (8) GloMax® Discover Microplate Reader | Plate Reader (8)](https://i0.wp.com/scv10mr-cdnpre-t-cus-00.azureedge.net/-/media/images/products/fluorometers-luminometers-multimode-readers/multimode-readers/glomax-discover/gm3000-luminescence-detection-multimode-plate-reader.jpg?rev=3cee10c1a52a435c807baf97119daf78&sc_lang=it-it)
- Plate mask (aperture control) for switching between 96-well and 384-well plates
- Low cross-talk between wells gives you better, more usable results
- Broad dynamic range of the instrument extends the linear range of your assay
![GloMax® Discover Microplate Reader | Plate Reader (9) GloMax® Discover Microplate Reader | Plate Reader (9)](https://i0.wp.com/scv10mr-cdnpre-t-cus-00.azureedge.net/-/media/images/products/fluorometers-luminometers-multimode-readers/multimode-readers/glomax-discover/gm3000-glomax-microplate-reader.jpg?rev=62807fb5f6db498cad035cc400c9967f&sc_lang=it-it)
- IQ/OQ Service available
- Provides the required technical elements of a part 11 compliant system when used with the appropriate laboratory workflow:
- User Authentication and Authorization
- Data Integrity and Protection
- Electronic Signatures
- Audit Trails
Accurate Results from Every Well
There are three ways that microplate readers can affect how much useful information you get from each plate. Poor sensitivity may cause you to miss low-level samples that could be the “hit” you are looking for. Letting the signal from bright wells spill into adjacent wells allows crosstalk to occur and skews your experimental results. And a narrow detection range limits the accuracy and reproducibility that your work requires. With the GloMax® plate readers, you can rest easy and have confidence in your results.
Glomax® microplate readers offer superior sensitivity and auto-gain adjustment letting you measure weak and strong signals on the same plate.
The masking design of GloMax® microplate readers means you can be confident that the results from each well are not skewed by signal from neighboring wells.
With the broad detection range of GloMax® microplate readers, you can measure signals ranging from high intensity to near physiological levels.
Easier, Faster Data Analysis with GloMax® Plate Readers
GloMax® plate readers have high sensitivity and a wide dynamic range, so you candetect both high and low signals on the same plate and have confidence in the accuracyof the signal recorded from each well. These instruments provide a range of optionsfor your throughput needs and are equipped with integrated software that makes dataanalysis easy. GloMax® instruments come preloaded with protocols designed to workseamlessly with a wide variety of Promega luminescent and fluorescence-based assaysat the touch of a button, including the Lumit™ Dx SARS-CoV-2 Immunoassay, CellTiter-Glo® Luminescent Cell Viability Assay, and the Nano-Glo®Luciferase Assay System.
No need to reinvent the wheel—we’ve already used GloMax® instruments for everything from cell viability, toxicity and metabolism, kinase and cell signaling, reporter gene assays, nucleic acid quantitation for NGS sequencing, PROTACs and protein degradation, bioassays, BRET, ELISAs and even water quality testing. Search our collection of protocols and data generated using a variety of different luminescent and fluorescence-based assays on the GloMax® Instruments.
Pre-installed Assay Protocols | ||||
---|---|---|---|---|
ADCC Reporter Bioassay | ADP-Glo | ApoLive-Glo | Apo-ONE | ApoTox-Glo |
Autophagy HiBiT Reporter Assay | BacTiter-Glo | BCA Protein Assay** | Bio-Glo | Bradford Assay** |
Bright-Glo | cAMP-Glo | Caspase-Glo | CellTiter 96 AQ ONE** | CellTiter-Blue |
CellTiter-Fluor | CellTiter-Glo | CellTiter-Green | Cholesterol/Cholesterol Ester-Glo | Chroma-Glo* |
Coumerin/Fluorescein Z-LYTE FRET* | CytoTox-Fluor | CytoTox-Glo | CytoTox-ONE | Dual Luciferase Assay |
Dual-Glo | ELISA (450nm)** | GFP Protein Quantitation | GloSensor cAMP | Glucose Uptake-Glo |
GTPase-Glo | Glycerol-Glo | Kinase-Glo | Lactate-Glo | LDH-Glo |
Luciferase Assay System | Lumit Immunoassay | Mitochondrial ToxGlo | MultiTox-Fluor | MultiTox-Glo |
NAD(P)H-Glo | NADP/NADPH-Glo | NanoBRET 618** | Nano-DLR | Nano-Glo® |
Nano-Glo® Endurazine | Nano-Glo® Live Cell | Nano-Glo® Live Cell (NanoBiT) | Nano-Glo® Vivazine | ONE-Glo |
ONE-Glo+Tox | P450-Glo | QuantiFluor dsDNA | QuantiFluor ONE dsDNA | QuantiFluor RNA |
QuantiFluor ssDNA | RealTime-Glo | RealTime-Glo Annexin V | Renilla Luciferase Assay System | Renilla-Glo |
Renilla / YFP BRET** | ROS-Glo | Steady-Glo | Succinate-Glo | Water-Glo |
* GloMax Discover only (GM3000)
** GloMax Explorer only (GM3500)
Not sure if GloMax® Discover is the right choice for your lab?
Find the instrument that's right for your needs.Compare Plate Readers
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Service and Support
One Call Supports It All
Promega supplies both the reagents and the instrument, so one call to Promega answers any questions you may have about assay chemistries or instrument performance.
Contact Technical Services
Ensure Minimal Instrument Downtime
- Field Support and Loaner Programs
- Service Packages
- IQ and OQ Packages
Warranty and Service Agreements
- Backed by a one-year warranty.
- Additional warranty and service agreements are available.
- For more information contact Promega Technical Services
Instrument Service and Support
Instrument Registration
![GloMax® Discover Microplate Reader | Plate Reader (15) GloMax® Discover Microplate Reader | Plate Reader (15)](https://i0.wp.com/scv10mr-cdnpre-t-cus-00.azureedge.net/-/media/images/products/fluorometers-luminometers-multimode-readers/multiplate-reader-fluorometer-support.jpg?rev=8e87ade1f27840b0845ac2ed4700ec36&sc_lang=it-it)
Design Features
Instrument Specifications
Detection Modes | Luminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET |
Sample Format | 6, 12, 24, 48, 96 and 384-well plates |
Tablet PC | Windows® 8.1 or 10 Tablet PC with USB connection to instrument |
Read Speed | 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes |
Automation Compatibility | Hardware and software accessible to 3rd party hardware/software control, including LIMS. |
Dimensions | 18.5in (46.9cm) width 19.5in (46.5cm) depth 10in (25.4cm) height without Tablet and PC mounted 16in (35.5cm) height with Tablet and PC mounted |
Weight | Approximately 60lbs. (27kg) |
Power Requirements | 120VA, 50/60 HZ |
Warranty | 1-year parts and labor warranty included |
Regulatory Compliance | For Research Use Only. Not for use in Diagnostic Procedures |
Luminescence Module Specifications
Detector | Top-reading, head-on photon counting photomultiplier tube (PMT) |
Wavelength Range | 350–700nm |
Detection Limit | 3 × 10-21moles of luciferase |
Linear Dynamic Range | 9 logs |
Cross-talk | Less than 3 × 10-5(white, 96-well plate, Corning 3912) |
Filtered Luminescence | Built-in filter paddle |
Fluorescence Module Specifications
Detector | PIN-photodiode |
Light Source | Wavelength-matched LED |
Read Position | Top reading |
Wavelengths selection | Filter module with 5 standard excitation and emission filters. Empty filter positions are available for custom excitation and emission wavelengths. |
Wavelengths included | UV (Ex: 365nm, Em: 415–445nm) Blue (Ex: 475nm, Em: 500–550nm) Green (Ex: 520nm, Em: 580–640nm) Red (Ex: 627nm, Em: 660–720nm) AFC (Ex: 405nm, Em: 495–505nm) |
Detection Limit | 2fmol fluorescence dye/200µl |
Linear Dynamic Range | >6 logs (assay dependent) |
UV-Visible Absorbance Module Specifications
Detector | Top-reading, head-on photon counting photomultiplier tube (PMT) |
Light Source | Xenon flash lamp |
Spectra Range | 200–600nm |
Filter Wheel | 9 absorbance filters included |
Wavelengths included | 230, 260, 280, 320, 405, 450, 490, 560 and 600nm 10nm band pass |
Detection Limit | 0.1 O.D. |
Dynamic Range | 0–4.0 O.D. |
Injector System Specifications
Number of injectors (optional) | Two injectors |
Dispense Volume Range | 5–200µl in 1µl increments |
Plate Compatibility | 6, 12, 24, 48, and 96-well plates |
Injection Speed | 20–500µl per second |
Waste Collection Tray Volume | Approximately 50ml |
Void Volume | 500µl |
Heating System Specifications
Temperature Range | Ambient to 45°C |
Shaker System Specifications
Shaking Pattern | Linear or orbital patterns |
Shaking Intensity | 100–500 cycles/minute |
PC Requirements
Operating System | Windows® 8.1 or 10 Pro, 64 bit, with USB connection to instrument |
RAM | 2GB |
Disk Space | 64GB |
Other Requirements | Microsoft.NET 4.0 Framework (full version), Microsoft Excel 2013 Professional |
Available Separately
Product | Catalog # |
---|---|
LuminometerLight Plate | E6531 |
GloMax® Discover Luminescence Filter Paddle | GM3011 |
GloMax® Discover Fluorescence Filter Paddle | GM3012 |
GloMax® Discover96 Half-Position Aperture Assembly | GM1050 |
GloMax® Dual Injectors with Pumps | GM3030 |
Inner and Outer Injector Tubing, Natural | GM3014 |
GloMax® Explorer Absorbance Module Upgrade | GM3520 |
GloMax® Discover-Explorer Sales Rental | E5301 |
GloMax® Discover or Explorer Installation Qualification | SA1104 |
GloMax® Discover or Explorer Operational Qualification | SA1105 |
GloMax® Discover or Explorer Installation and Operational Qualification | SA1106 |
GloMax® Discover Standard Service Agreement | SA4000 |
GloMax® Discover or ExplorerPreventive Maintenance | SA4030 |
Protocols
Complete Protocol
GloMax® Discover SystemPDF (28536 KB) – English
Quick Protocols
GloMax® Discover System Quick Start GuidePDF (3036 KB) – English
GloMax Discover System Creating a New Protocol FB166PDF (450 KB) – English
Video Protocols
Specifications
SDS
Search for SDS
Certificate of Analysis
Search by lot number
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
Patent Pending.
Resources
Citations
-
β-Arrestin1 and 2 differentially regulate PACAP-induced PAC1 receptor signaling and trafficking.
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2018 PLos ONE - See all citations
Software and Firmware
- GloMax® Discover
- Protocols for GloMax® Discover and GloMax® Explorer Systems
Other
- GloMax® Application Notes
- How To Export Templates and Results Using the GloMax® Analysis Software
- How To Edit a Graph Using the GloMax® Analysis Software
- How To Create an Analysis Template Using the GloMax® Analysis Software
- Measuring BRET Assays on the GloMax® Discover System
- Replacing Injector Tubing on GloMax Discover or Explorer Microplate Reader
- GloMax® Access Panel Installation Video
- GloMax® Access Panel Removal Video